The Human Myosin Light Chain Kinase (MLCK) from Hippocampus: Cloning, Sequencing, Expression, and Localization to 3qcen–q21
Identifieur interne : 000330 ( France/Analysis ); précédent : 000329; suivant : 000331The Human Myosin Light Chain Kinase (MLCK) from Hippocampus: Cloning, Sequencing, Expression, and Localization to 3qcen–q21
Auteurs : M.-C. Potier [France] ; E. Chelot [France] ; Y. Pekarsky ; K. Gardiner ; J. Rossier [France] ; W. G. Turnell [France]Source :
- Genomics [ 0888-7543 ] ; 1995.
English descriptors
- Teeft :
- Amino, Amino acid sequence, Biol, Bovine, Broblast, Carboxyl, Carboxyl terminus, Catalytic domain, Cdna, Cell biol, Cell lines, Chem, Chromosome, Clone, Clone mlck1, Codon, Ecori, Entorhinal cortex, Frontal cortex, Gallagher, Genomic, Genomic sequence, Gizzard, Gnma, Hamster, High stringency, Hippocampus, Human brain mlck, Human brain mlck cdna, Human chromosome, Human genomic, Human mlck, Hybridized, Independent protein, Intact chromosome, Kinase, Kobayashi, Localization, Mlck, Mlck1, Mlck2a, Mlcks, Mrna, Myosin, Myosin light chain kinase, Neuron, Nonmuscle, Nonmuscle mlcks, Northern blot analysis, Nucleotide, Open reading frame, Primer, Skeletal muscle, Smmlck, Smooth muscle, Somatic cell, Telokin, Turkey gizzard.
Abstract
Abstract: Myosin light chain kinase (MLCK), a key enzyme in muscle contraction, has been shown by immunohistology to be present in neurons and glia. We describe here the cloning of the cDNA for human MLCK from hippocampus, encoding a protein sequence 95% similar to smooth muscle MLCKs but less than 60% similar to skeletal muscle MLCKs. The cDNA clone detected two RNA transcripts in human frontal and entorhinal cortex, in hippocampus, and in jejunum, one corresponding to MLCK and the other probably to telokin, the carboxy-terminal 154 codons of MLCK expressed as an independent protein in smooth muscle. Levels of expression were lower in brain compared to smooth muscle. We show that within the protein sequence, a motif of 28 or 24 residues is repeated five times, the second repeat ending with the putative methionine start codon. These repeats overlap with a second previously reported module of 12 residues repeated five times in the human sequence. In addition, the acidic C-terminus of all MLCKs from both brain and smooth muscle resembles the C-terminus of tubulins. The chromosomal localization of the gene for human MLCK is shown to be at 3qcen–q21, as determined by PCR and Southern blotting using two somatic cell hybrid panels.
Url:
DOI: 10.1006/geno.1995.9965
Affiliations:
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Turnell</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut Alfred Fessard, Centre National de la Recherche Scientifique, 91198 Cedex, Gif-sur-Yvette</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Gif-sur-Yvette</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Genomics</title><title level="j" type="abbrev">YGENO</title><idno type="ISSN">0888-7543</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">29</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="562">562</biblScope><biblScope unit="page" to="570">570</biblScope></imprint><idno type="ISSN">0888-7543</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0888-7543</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid sequence</term><term>Biol</term><term>Bovine</term><term>Broblast</term><term>Carboxyl</term><term>Carboxyl terminus</term><term>Catalytic domain</term><term>Cdna</term><term>Cell biol</term><term>Cell lines</term><term>Chem</term><term>Chromosome</term><term>Clone</term><term>Clone mlck1</term><term>Codon</term><term>Ecori</term><term>Entorhinal cortex</term><term>Frontal cortex</term><term>Gallagher</term><term>Genomic</term><term>Genomic sequence</term><term>Gizzard</term><term>Gnma</term><term>Hamster</term><term>High stringency</term><term>Hippocampus</term><term>Human brain mlck</term><term>Human brain mlck cdna</term><term>Human chromosome</term><term>Human genomic</term><term>Human mlck</term><term>Hybridized</term><term>Independent protein</term><term>Intact chromosome</term><term>Kinase</term><term>Kobayashi</term><term>Localization</term><term>Mlck</term><term>Mlck1</term><term>Mlck2a</term><term>Mlcks</term><term>Mrna</term><term>Myosin</term><term>Myosin light chain kinase</term><term>Neuron</term><term>Nonmuscle</term><term>Nonmuscle mlcks</term><term>Northern blot analysis</term><term>Nucleotide</term><term>Open reading frame</term><term>Primer</term><term>Skeletal muscle</term><term>Smmlck</term><term>Smooth muscle</term><term>Somatic cell</term><term>Telokin</term><term>Turkey gizzard</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Myosin light chain kinase (MLCK), a key enzyme in muscle contraction, has been shown by immunohistology to be present in neurons and glia. We describe here the cloning of the cDNA for human MLCK from hippocampus, encoding a protein sequence 95% similar to smooth muscle MLCKs but less than 60% similar to skeletal muscle MLCKs. The cDNA clone detected two RNA transcripts in human frontal and entorhinal cortex, in hippocampus, and in jejunum, one corresponding to MLCK and the other probably to telokin, the carboxy-terminal 154 codons of MLCK expressed as an independent protein in smooth muscle. Levels of expression were lower in brain compared to smooth muscle. We show that within the protein sequence, a motif of 28 or 24 residues is repeated five times, the second repeat ending with the putative methionine start codon. These repeats overlap with a second previously reported module of 12 residues repeated five times in the human sequence. In addition, the acidic C-terminus of all MLCKs from both brain and smooth muscle resembles the C-terminus of tubulins. The chromosomal localization of the gene for human MLCK is shown to be at 3qcen–q21, as determined by PCR and Southern blotting using two somatic cell hybrid panels.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Gif-sur-Yvette</li></settlement></list><tree><noCountry><name sortKey="Gardiner, K" sort="Gardiner, K" uniqKey="Gardiner K" first="K." last="Gardiner">K. Gardiner</name><name sortKey="Pekarsky, Y" sort="Pekarsky, Y" uniqKey="Pekarsky Y" first="Y." last="Pekarsky">Y. Pekarsky</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Potier, M C" sort="Potier, M C" uniqKey="Potier M" first="M.-C." last="Potier">M.-C. Potier</name></region><name sortKey="Chelot, E" sort="Chelot, E" uniqKey="Chelot E" first="E." last="Chelot">E. Chelot</name><name sortKey="Rossier, J" sort="Rossier, J" uniqKey="Rossier J" first="J." last="Rossier">J. Rossier</name><name sortKey="Turnell, W G" sort="Turnell, W G" uniqKey="Turnell W" first="W. G." last="Turnell">W. G. Turnell</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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